Runella defluvii sp. nov., isolated from a domestic wastewater treatment plant

Abstract

A long, Gram-negative, rod-shaped bacterium, designated strain EMB13 T , was isolated from a wastewater treatment plant in Korea. The isolate was strictly aerobic and non-motile. The strain grew optimally at 30–35 °C and pH 7.5–8.0, and the predominant fatty acids were iso-C 15 : 0 , summed feature 3 (C 16 : 1 ω 7 c and/or iso-C 15 : 0 2-OH), C 16 : 1 ω 5 c and iso-C 17 : 0 3-OH. The strain contained a large amount of phosphatidylethanolamine and small amounts of phosphatidylcholine and an unknown phospholipid as the polar lipids. The G+C content of the genomic DNA was 40.1 mol% and the major isoprenoid quinone was menaquinone-7. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB13 T belonged to the genus Runella and was most closely related to Runella limosa EMB111 T , with a 16S rRNA gene sequence similarity of 97.1 %. DNA–DNA relatedness between strain EMB13 T and R. limosa EMB111 T was approximately 25 %. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain EMB13 T represents a novel species within the genus Runella , for which the name Runella defluvii sp. nov. is proposed. The type strain is EMB13 T (=KCTC 12614 T =DSM 17976 T ).