Concepedia

Utilization of Hydrophilic-Interaction Lc to Minimize Matrix Effects Caused By Phospholipids

Beijing Tan, Andre Negahban, Thomas S. McDonald, Yizhong Zhang, Christopher L. Holliman

Bioanalysis · 2012 · 23 citations · 17 references

DOI

Abstract

HILIC chromatography is a valued tool in the development of robust bioanalytical assays with minimal and predictable phospholipid interferences. Furthermore, addition of a small amount of ion-pairing additives can reliably move pharmaceutical compounds away from these suppressive regions.

References

17

Strategies for the Assessment of Matrix Effect in Quantitative Bioanalytical Methods Based on HPLC−MS/MS

B.K. Matuszewski, M.L. Constanzer, C.M. Chavez-Eng · Analytical Chemistry · 2003 · 5.1K citations

Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds

Andrew J. Alpert · Journal of Chromatography A · 1990 · 1.9K citations

Matrix Effect in Quantitative LC/MS/MS Analyses of Biological Fluids:  A Method for Determination of Finasteride in Human Plasma at Picogram Per Milliliter Concentrations

B.K. Matuszewski, M.L. Constanzer, C.M. Chavez-Eng · Analytical Chemistry · 1998 · 873 citations

, , +21

Systematic and comprehensive strategy for reducing matrix effects in LC/MS/MS analyses

Erin Chambers, Diane M. Diehl, Ziling Lu et al. · Journal of Chromatography B · 2007 · 738 citations

, , +5

Liquid chromatography–mass spectrometry/mass spectrometry method development for drug metabolism studies: Examining lipid matrix ionization effects in plasma

Julian Little, Michael F. Wempe, Charles Buchanan · Journal of Chromatography B · 2006 · 253 citations

, , +10