Naphthalene Degradation Kinetics of <i>Micrococcus</i> sp., Isolated from Activated Sludge

Abstract

Abstract Biodegradation of naphthalene by Micrococcus sp., isolated from the effluent of an activated sludge plant, was studied. The effects of pH (5–8), glucose concentration (100–1000 mg/L) and inoculum concentrations (1–5%) on the growth and naphthalene degradation potential of Micrococcus sp. were investigated. Maximum naphthalene degradation and subsequent high microbial growth were observed at optimum pH (pH 7), glucose concentration (500 mg/L) and inoculum concentration (3%). To investigate the maximum naphthalene tolerance potential of Micrococcus sp., very high concentrations of naphthalene (500–5000 mg/L) were used in the presence of non‐ionic surfactants. The examined surfactants (Triton X‐100 and Tween‐80) increased the bioavailability of naphthalene to the microbes and Complete naphthalene degradation by Micrococcus sp. was observed at an initial naphthalene concentration of 500 mg/L. However, the degradation potential decreases as the naphthalene concentration increases. Very high naphthalene concentrations also affected the growth of microbes and the corresponding substrate inhibition kinetics was described using four models (Haldane, Webb, Edward and Aiba). Based on correlation coefficient and percentage error values, all four substrate kinetic models were able to describe the dynamic behavior of naphthalene biodegradation by Micrococcus sp.