The effects of salt stress on growth, nitrate reduction and proline and glycinebetaine accumulation in Prosopis alba

Abstract

Prosopis alba (algarrobo) is one of the most important salt-tolerant legumes used in the food and furniture industries. The effects of salinity on some growth and physiological parameters in algarrrobo seedlings were investigated. 17-Day-old seedlings were subjected to three salt treatments by adding NaCl to the growth medium in 50 mmol.L-1 increments every 24 h until the final concentrations of 0, 300 and 600 mmol.L-1 were reached. Only the highest NaCl concentration affected all of the considered parameters. Thus, 600 mmol.L-1 NaCl caused a significant reduction in root and shoot growth, but an increase in the root/shoot ratio. Leaf relative water content, nitrate content and nitrate reductase activity in leaves and roots were also decreased. At 300 and 600 mmol.L-1, the glycinebetaine content was significantly increased in both leaves and roots but this was not found for proline content. Total soluble carbohydrates increased only in roots. The results suggest that glycinebetaine enhancement may be important for osmotic adjustment in Prosopis alba under salinity stress.