Glutathione S-transferase and Catalase gene polymorphisms with Type 2 diabetes mellitus.

Abstract

Background and Aim: Antioxidant enzymes such as glutathione S-transferases (GSTs) and catalase (CAT) play important roles in cellular defense by detoxifying various toxic substrates and can be used as important biomarkers for T2DM. The aim of the present work was to study the association of GST and CAT gene polymorphism with T2DM cases and controls in north Indian population. Materials and Methods: Polymorphic GST gene isoforms, GSTM1, T1 and P1 were investigated in 201 healthy control subjects and 204 T2DM cases while CAT -21A/T polymorphism in 186 controls and 189 T2DM cases. Genotypes were analyzed by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genotype distribution and allelic frequencies were compared between patients and controls. Mean values and odds ratio (OR) with 95% confidence interval (CI) were calculated using SPSS software (version 15.0). Results: Biochemical parameters showed highly significant association with T2DM (P