Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition

Andrew Adey, Hilary G. Morrison, Asan, Xun Xu, Jacob O. Kitzman, Emily H. Turner, Bethany Stackhouse, Alexandra P MacKenzie, Nicholas Caruccio, Xiuqing Zhang,

Genome biology · 2010 · 622 citations · 22 references

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Abstract

We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing.

References

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