Brevundimonas aveniformis sp. nov., a stalked species isolated from activated sludge

Abstract

A Gram-negative, rod-like, stalk-producing bacterium, designated strain EMB102(T), was isolated from activated sludge that performed enhanced biological phosphorus removal in a sequencing batch reactor. Cells without stalks were motile with single polar flagella, but cells that did produce stalks were non-motile and lacked polar flagella. Growth of strain EMB102(T) was observed at temperatures between 15 and 35 degrees C (optimum, 30 degrees C) and between pH 6.0 and 9.0 (optimum, pH 7.5-8.5). The predominant fatty acids of strain EMB102(T) were C(18 : 1) omega 7c, C(16 : 0) and C(15 : 0). The predominant polar lipid was phosphatidylglycerol. The G+C content of the genomic DNA was 64.1 mol% and the major quinone was Q-10. Comparative 16S rRNA gene sequence analyses showed that strain EMB102(T) formed a distinct phyletic lineage within the genus Brevundimonas. The levels of 16S rRNA gene sequence similarity between the type strains of Brevundimonas species ranged from 95.8 to 97.5 %. DNA-DNA relatedness levels between the EMB102(T) and closely related Brevundimonas species were below 15.0 %. On the basis of chemotaxonomic data and molecular properties, strain EMB102(T) represents a novel species within the genus Brevundimonas, for which the name Brevundimonas aveniformis sp. nov. is proposed. The type strain is EMB102(T) (=KCTC 12609(T)=DSM 17977(T)).