Bryostatin 1 antagonizes the terminal differentiating action of 12-<i>O</i>-tetradecanoylphorbol-13-acetate in a human colon cancer cell

Abstract

12-O-Tetradecanoylphorbol-13-acetate (TPA), a highly active representative of the tumor-promoting phorbol esters, induces a rapid terminal differentiation of a human colon cancer cell line. Bryostatin 1, a macrocyclic lactone, completely counteracts this effect of TPA and promotes continued replication. The observed responses provide a system for identifying cellular processes which are involved in the induced terminal differentiation of human colon cancer cells.