Production of Enterocin P, an Antilisterial Pediocin-Like Bacteriocin from <i>Enterococcus faecium</i> P13, in <i>Pichia pastoris</i>

Abstract

The gene encoding mature enterocin P (EntP), an antimicrobial peptide from Enterococcus faecium P13, was cloned into the pPICZalphaA expression vector to generate plasmid pJC31. This plasmid was integrated into the genome of P. pastoris X-33, and EntP was heterologously secreted from the recombinant P. pastoris X-33t1 derivative at a higher production and antagonistic activity than from E. faecium P13.