LCMS-QTOF Determination of Lentinan-Like <b><i>β</i></b>-D-Glucan Content Isolated by Hot Water and Alkaline Solution from Tiger’s Milk Mushroom, Termite Mushroom, and Selected Local Market Mushrooms

Abstract

Lentinan, 1152 Dalton β -D-glucan found in Shiitake Mushroom ( Lentinus edodes ), has been claimed to have anticancer and immunomodulatory activity. Several extraction methods have been used by researchers to isolate Lentinan including hot water and alkaline solution (1.25 M NaOH). In this study, hot water and alkaline solution (1.25 M NaOH) were used to extract the Lentinan-like β -D-glucan (1151 Dalton) from Tiger’s Milk Mushroom, Termite Mushroom, and selected local market mushrooms. The isolated Lentinan-like β -D-glucan from both hot water and alkaline solution was analyzed by LCMS-QTOF. Commercial Lentinan standard from Lentinus edodes was used as a reference. The results showed significant differences on chromatogram patterns of Lentinan-like β -D-glucan between both extracts. The peak of Lentinan-like β -D-glucan was only found in isolated polysaccharide glucan of hot water extracts. The isolated polysaccharide glucans from Tiger’s Milk Mushroom and Termite Mushroom were found to have <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mn>0.74</mml:mn><mml:mo>±</mml:mo><mml:mn>0.12</mml:mn></mml:math> μ g/mg and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mn>0.53</mml:mn><mml:mo>±</mml:mo><mml:mn>0.07</mml:mn></mml:math> μ g/mg Lentinan-like β -D-glucan. Button Mushroom, Shiitake Mushroom, and Oyster Mushroom showed the presence of Lentinan-like β -D-glucan at <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mn>16.16</mml:mn><mml:mo>±</mml:mo><mml:mn>4.15</mml:mn></mml:math> μ g/mg, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mn>0.22</mml:mn><mml:mo>±</mml:mo><mml:mn>0.04</mml:mn></mml:math>, and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M5"><mml:mn>0.10</mml:mn><mml:mo>±</mml:mo><mml:mn>0.01</mml:mn></mml:math> μ g/mg, respectively.