Human Gene Therapy · 1999 · 259 citations · 12 references
EngineeringSynthetic VirologyMolecular BiologyGene DeliveryVirus GeneE1/e4-deleted VectorViral GeneticsGene TransferVitro Ligation MethodSimple MethodVirologyCell BiologyBiomolecular EngineeringVector ConstructionSynthetic BiologyGenetic EngineeringGene VectorMedicineViral OncologyGenome Editing
We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.
12
Andrew J. Bett, Wael Haddara, L. Prevec et al. · Proceedings of the National Academy of Sciences · 1994 · 742 citations · Full text