Concepedia

Design and Application of a New Cryptic-Plasmid-Based Shuttle Vector for <i>Magnetospirillum magneticum</i>

Yoshiko Okamura, Haruko Takeyama, Takumi Sekine, Toshifumi Sakaguchi, Aris Tri Wahyudi, Rika Sato, Shinji Kamiya, Tadashi Matsunaga

Applied and Environmental Microbiology · 2003 · 54 citations · 19 references

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Abstract

A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed.

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