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Substrate Distortion by a -Mannanase: Snapshots of the Michaelis and Covalent-Intermediate Complexes Suggest a B2,5 Conformation for the Transition State
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2002
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GlycobiologyMolecular BiologyPolysaccharideChemistryTransition StateBiosynthesisStructure-function Enzyme KineticsGlycosylationSubstrate DistortionBiochemistryConformational SpaceConformational StudyQuantum ChemistryMolecular ModelingStructural BiologySubstrate DistortionsNatural SciencesConformational Reaction PathwayMolecular ComplexMedicineCarbohydrate-protein InteractionB2,5 Conformation
The conformational reaction pathway for β-mannosidases proposed here is distinct from that of glucosidases and cellulases. The proposal is based on substrate distortions along the reaction pathway of a β-mannosidase (see picture) that were revealed by X-ray crystallography and are close in conformational space to known β-mannosidase inhibitors.