Proceedings of the Royal Society of London. Series B, Biological sciences · 1973 · 131 citations · 9 references
Life SciencesBiochemistryAgingLipid BiochemistryReproductive BiologyLipid OxidationLipid PeroxidationMale Reproductive FunctionOxidative Damage To BiomoleculesSperm BiologySperm FunctionHomogenizationOxidative Damage To SpermatozoaOxidative Stress - Induced Membrane DamagePeroxide FormationSeminal Plasma Oxidative StressSperm Oxidative StressSperm Reactive Oxygen SpeciesThiobarbituric Acid Reactive Substances
Abstract Ram spermatozoa produce aerobically an organic peroxide which can be determined quantitatively by the thiobarbituric acid reaction. The reaction is more intense in spermatozoa that have been stored at 5°C. Cold shock and homogenization release from spermatozoa a substance, presumably a lipid, which provides the substrate for peroxidation. Lipid peroxidation is probably linked with the decline in motility and respiratory activity, and the structural damage to sperm membranes, which characterize ageing or degenerating spermatozoa.
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The Biochemistry of Semen and of the Male Reproductive Tract
The Medical Journal of Australia · 1965 · 1.7K citations
Lipide peroxidation in isolated mitochondria
Al L. Tappel, H Zalkin · Archives of Biochemistry and Biophysics · 1959 · 313 citations