Concepedia

Modified Multiplex PCR Method for Detection of Pyrogenic Exotoxin Genes in Staphylococcal Isolates

Astrid Løvseth, Semir Lončarević, Knut G Berdal

Journal of Clinical Microbiology · 2004 · 181 citations · 17 references

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Abstract

A modified multiplex PCR method for detection of nine Staphylococcus aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and one form of immunoreactive toxic shock syndrome toxin based on a previously published method (S. R. Monday and G. A. Bohach, J. Clin. Microbiol. 37:3411-3414, 1999) has been developed. The modified PCR protocol seems robust and gives reliable results.

References

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Use of Multiplex PCR To Detect Classical and Newly Described Pyrogenic Toxin Genes in Staphylococcal Isolates

Steven R. Monday, Gregory A. Bohach · Journal of Clinical Microbiology · 1999 · 389 citations · Full text

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Detection of <i>seg</i> , <i>seh</i> , and <i>sei</i> genes in <i>Staphylococcus aureus</i> Isolates and Determination of the Enterotoxin Productivities of <i>S. aureus</i> Isolates Harboring <i>seg</i> , <i>seh</i> , or <i>sei</i> Genes

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