Marine Drugs · 2004 · 59 citations · 15 references
Natural SciencesMarine SciencesMedicinal ChemistryDrug DiscoveryMarine BiotechnologyMarine ParasitologyAntiparasitic AgentsEnzyme InhibitionMarine Bioactive CompoundsMarine Drug DiscoveryMarine PharmacologyActive Compound IsolationAntileishmanial TreatmentAssayBioactive Compounds From Marine SpongesBioassay-guided FractionationDrug Screening AssaysMarine Compound ScreeningMarine Sponge Metabolites
The newly developed assay system using recombinant Leishmania amazonensis expressing enhanced green fluorescent protein (La/egfp) has been applied to the screening of Japanese marine sponges for antileishmanial activity. Bioassay-guided fractionation of an active sponge Neopetrosia sp. afforded an active compound which was identified as renieramycin A by spectroscopic analysis. It inhibited La/egfp with an IC50 value of 0.2 μg/mL.
15