Ground Water · 1986 · 42 citations · 12 references
Life SciencesEnvironmental SciencesMicrobiologyBacteriologyEnvironmental MicrobiologyMicrobial EcologyBacterial EcologyBacterial GrowthBacterial MetabolismEnvironmental BacteriologyMicrobial GrowthWater Quality MicrobiologyAcridine Orange TestAquatic Bacterial EcologyHeterotrophic Metabolism
ABSTRACT Ground water from deep wells was sampled for the presence of heterotrophic bacteria. Over 500 bacteria were isolated from well‐water samples on a low‐nutrient medium (R2A). Gram‐negative, rod‐shaped, nonmotile bacteria predominated, and Acinetobacter spp. comprised 54% of the total number of isolates. Selected isolates were inoculated into unamended and carbon‐enriched well water, and growth was measured by acridine orange direct count (AODC). Carbon sources included glucose, acetate, pyruvate, and succinate in 100 μg carbon/liter and 1,000 μg carbon/liter concentrations. The isolates grew in unamended filtered well water within 24 hours, and growth of an Acinetobacter sp. was further stimulated (greater than two orders of magnitude within five days) in the carbon‐enriched well water.
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