Acute and subchronic toxicity study of the water extract from root of <i>Sida rhombifolia</i> Linn. in rats

Abstract

Acute and subchronic toxicities of the water extract from the root of <i>Sida rhombifolia</i> Linn. were studied in both male and female rats. Oral administration of the extract at a single dose of 5,000 mg/kg body weight (5 males, 5 females) did not produce signs of toxicity, behavioral changes, mortality or differences on gross appearance of internal organs. The subchronic toxicity was determined by oral feeding the test substance at the doses of 300, 600 and 1,200 mg/kg body weight for 90 days (10 males, 10 females). The examinations of signs, animal behavior and health monitoring showed no signs of abnormalities in the test groups as compared to the controls. The test and control groups (on the 90^th day) and the satellite group (on the 118^th day) were analyzed by measuring their final body and organ weights, taking necropsy, and examining hematological parameters, blood clinical chemistry and histopathology features. The results suggest that <i>S. rhombifolia</i> administered orally did not cause acute or subchronic toxicities to male and female rats.