Effects of phytoplankton bloom in a coastal ecosystem on the composition of bacterial communities

Abstract

We studied the composition of free-living and particle-associated bacterial communities during the course of the phytoplankton succession in spring and early summer in the Wadden Sea, a tidal flat ecosystem in the southern North Sea. We applied the denaturing gradient gel electrophoresis (DGGE) approach based on PCR-amplified 16S rRNA gene fragments and, in addition to Bacteriaspecific primers, used primer sets targeting Alphaproteobacteria, the Roseobacter-clade and the Bacteroidetes phylum. Even though the application of the primer sets targeting Bacteria and Alphaproteobacteria detected some changes, they were most pronounced with those targeting the Roseobacter-clade and Bacteroidetes. The changes were supported by a correspondence analysis, which showed a statistically significant correlation of the DGGE banding patterns of the Roseobacter-specific PCR with the composition of the phytoplankton (p = 0.03). This indicates that changes in the phytoplankton composition in this habitat are not reflected by changes in the most abundant or most readily amplifiable phylotypes. The findings rather suggest that few, specialized, heterotrophic bacteria are most responsive to the organic matter supplied by distinct phytoplankton communities and that the main part of organic matter in the Wadden Sea is utilized by generalists. Sequence analyses of excised bands revealed a high diversity for the Bacteriaand Bacteroidetes-targeted approaches. The bacterial community detected by the primer set targeting Alphaproteobacteria, however, was mainly composed of bacteria affiliated to the Roseobacter-clade.