ChemBioChem · 2002 · 194 citations · 0 references
Life SciencesBiochemistryMolecular BiologyBiosensorsEnzyme TechnologyEnzymologyBiosensor DesignDna BiosensorsEnzymatic BiosensorsEnzyme-based BiosensorsEnzyme-substrate RecognitionMacromolecular AssemblyBiotin-streptavidin ImmobilizationDna Hybridization AssaysDna Hybridization BiosensorsEnzyme Process DesignOxidoreductase CatalysisOxidoreductase EnzymesSchematic RepresentationSignal Amplification Mechanisms
Spatially controlled assembly of enzyme conjugates can be applied to signal amplification systems for biosensors, or synthesis of novel catalysts for enzyme process technology. Artificial bienzymic systems comprised of two enzymes, NAD(P)H-FMN oxidoreductase and luciferase (represented as blue and orange spheres, respectively, in the schematic representation) were assembled by means of DNA hybridization and streptavidin–biotin interaction.