Rapid Production of Gene Replacement Constructs and Generation of a Green Fluorescent Protein-Tagged Centromeric Marker in <i>Aspergillus nidulans</i>

Abstract

ABSTRACT A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans . The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans .