Adaptation of nutrient supply to fetal demand in the mouse involves interaction between the Igf2 gene and placental transporter systems

TLDR

The mammalian fetus relies on maternal nutrient supply through the placenta, requiring a finely tuned balance between maternal supply and fetal demand to ensure healthy growth, with imbalances potentially causing long‑term health consequences. Our study shows that loss of placental Igf2 triggers up‑regulation of glucose and amino‑acid transporters (Slc2a3, Slc38a4) to meet fetal demand, while reducing fetal Igf2 eliminates this response, demonstrating that Igf2 signals modulate placental transporter expression and that this crosstalk may govern nutrient supply during development.

Abstract

The mammalian fetus is unique in its dependence during gestation on the supply of maternal nutrients through the placenta. Maternal supply and fetal demand for nutrients need to be fine tuned for healthy growth and development of the fetus along its genetic trajectory. An altered balance between supply and demand can lead to deviations from this trajectory with long-term consequences for health. We have previously shown that in a knockout lacking the imprinted placental-specific Igf2 transcript (P0), growth of the placenta is compromised from early gestation but fetal growth is normal until late gestation, suggesting functional adaptation of the placenta to meet the fetal demands. Here, we show that placental transport of glucose and amino acids are increased in the Igf2 P0 +/- null and that this up-regulation of transport occurs, at least in part, through increased expression of the transporter genes Slc2a3 and Slc38a4 , the imprinted member of the System A amino acid transporter gene family. Decreasing fetal demand genetically by removal of fetal Igf2 abolished up-regulation of both transport systems and reduced placental System A amino acid transport activity and expression of Slc38a2 in late gestation. Our results provide direct evidence that the placenta can respond to fetal demand signals through regulation of expression of specific placental transport systems. Thus, crosstalk between an imprinted growth demand gene ( Igf2 ) and placental supply transporter genes ( Slc38a4 , Slc38a2 , and Slc2a3 ) may be a component of the genetic control of nutrient supply and demand during mammalian development.