Cloning, Sequencing, and Expression in <i>Escherichia coli</i> of the New Gene Encoding β-1,3-Xylanase from a Marine Bacterium, <i>Vibrio</i> sp. Strain XY-214

Abstract

The Vibrio sp. strain XY-214 beta-1,3-xylanase gene cloned in Escherichia coli DH5alpha consisted of an open reading frame of 1, 383 nucleotides encoding a protein of 460 amino acids with a molecular mass of 51,323 Da and had a signal peptide of 22 amino acids. The transformant enzyme hydrolyzed beta-1,3-xylan to produce several xylooligosaccharides.