Concepedia

Lipid‐Anchored Oligonucleotides for Stable Double‐Helix Formation in Distinct Membrane Domains

Anke Kurz, Andreas Bunge, Anne‐Katrin Windeck, Maximilian Rost, Wolfgang Flasche, Anna Arbuzova, Denise Strohbach, Sabine Müller, Jürgen Liebscher, Daniel Huster,

Angewandte Chemie International Edition · 2006 · 82 citations · 19 references

DOI

Concepts

Abstract

Anchoring for DNA: A lipophilic oligonucleotide consisting of 21 thymidine units and two lipophilic nucleotides L was synthesized and bound to vesicular surfaces (see fluorescence image). The membrane-bound oligonucleotide binds complementary DNA strands by formation of Watson–Crick base pairs. The lipid-anchored oligonucleotide is preferentially enriched in liquid-disordered membrane domains.

References

19

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Kirsten Bacia, Petra Schwille, Teymuras V. Kurzchalia · Proceedings of the National Academy of Sciences · 2005 · 411 citations · Full text