Applied and Environmental Microbiology · 1999 · 64 citations · 23 references
Life SciencesBiochemistryPlant BiologyEnzymologyPlant BiochemistryPlant-microbe InteractionsEnzyme InhibitionEthylene BiologyPhenylpropanoid BiosynthesisPlant EnzymesPlant Natural Product Biosynthesis1-Aminocyclopropane-1-carboxylic Acid SynthaseAssayAssay SensitivityAssay SpecificityBotanical BioassaysEthylene Biosynthesis InhibitionEthylene InhibitionRhizobial Symbiosis
Rhizobitoxine is synthesized by the legume symbiont Bradyrhizobium elkanii and the plant pathogen Burkholderia andropogonis. Rhizobitoxine competitively inhibited 1-aminocyclopropane-1-carboxylate (ACC) synthase bLE-ACS2 from the tomato, a key enzyme in the pathway of ethylene biosynthesis. Based on this inhibition of ACC synthase, we have developed a new assay for rhizobitoxine.
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