Construction of a Broad-Host-Range Tn <i>7</i> -Based Vector for Single-Copy P _BAD -Controlled Gene Expression in Gram-Negative Bacteria

Abstract

We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the P(BAD) promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.