Infection and Immunity · 2002 · 77 citations · 25 references
Life SciencesMicrobiologyMolecular BiologyComparative MicrobiologyHost-pathogen InteractionsProteomicsBacterial PathogenesisBacterial Virulence FactorsComparative PathogenesisMicrobial ProteomicsMicrobial Virulence FactorsPathogen Virulence FactorsPathogen ProteomicsPolymerase Chain Reaction (Pcr) AmplificationProteomic IdentificationVirulence Factor IdentificationVirulence Gene AnalysisVirulence Gene DetectionVirulence Gene IdentificationVirulence Proteomics
A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.
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Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
Andrej Shevchenko, Matthias Wilm, Ole Vorm et al. · Analytical Chemistry · 1996 · 9.1K citations
Michael Hensel, Jacqueline E. Shea, Scott R. Waterman et al. · Molecular Microbiology · 1998 · 605 citations · Full text