Construction of p16S <i>lux</i> , a Novel Vector for Improved Bioluminescent Labeling of Gram-Negative Bacteria

Abstract

A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.