Proceedings of the National Academy of Sciences · 1982 · 1.4K citations · 29 references
Life SciencesGeneticsMolecular BiologyCytogeneticsMolecular OncologyOncogenesChromosomal TranslocationsImmunoglobulin Gene RearrangementLymphoid NeoplasmsLymphoid TumorsMyc Family OncogenesNeoplastic TransformationImmunoglobulin Heavy Chain LocusImmunoglobulin Heavy Chain RearrangementImmunoglobulin Light Chain LocusMolecular Mechanisms Of TransformationMyc In Burkitt LymphomaOncogenic TransformationReciprocal TranslocationsTranslocations
Chromosomal translocations are consistently observed in Burkitt lymphoma and murine plasmacytoma, implying a potential role in malignant transformation. We demonstrate that c‑myc is frequently translocated into immunoglobulin loci—into the Igμ gene in human Burkitt cells and into the Igα switch region in mouse plasmacytoma—providing a molecular basis for the role of these translocations in malignant transformation.
The consistent appearance of specific chromosomal translocations in human Burkitt lymphomas and murine plasmacytomas has suggested that these translocations might play a role in malignant transformation. Here we show that transformation of these cells is frequently accompanied by the somatic rearrangement of a cellular analogue of an avian retrovirus transforming gene, c-myc. Moreover, we map c-myc to human chromosome 8 band q24, the chromosomal segment involved in the reciprocal Burkitt translocations [t(8;14), t(8;22) and t(2;8)]. In two t(8;14) human Burkitt cell lines, c-myc appears to have been translocated directly into a DNA restriction fragment that also encodes the immunoglobulin mu chain gene. In the case of a specific cloned fragment of DNA derived from a mouse plasmacytoma, we demonstrate directly that c-myc has been translocated into the immunoglobulin alpha switch region. Our data provide a molecular basis for considering the role that specific translocations might play in malignant transformation.
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