Memórias do Instituto Oswaldo Cruz · 1991 · 13 citations · 0 references
AgricultureAgronomyPlant SciencesBreeding For Disease ResistanceCrop ProtectionPlant-microbe InteractionsPest ResistancePlant Disease ResistanceResistance ManagementResistant Crop VarietiesSeed Pathogen ResistanceEntomological InvestigationProteolytic Enzyme ActivationProteolytic Enzyme ActivityProteolytic Enzyme SpecificityResistance Gene MechanismsResistance To Seed-borne PathogensSeed ImmunitySeed Lectins
Cowpea (Vigna unguiculata) seeds are heavily damaged during storage by the bruchid Callosobruchus maculatus. Seeds of some Nigerian varieties showed a strong resistance to this bruchid. By utilizing biochemical and entomological techniques we were able to rule out the paticipation of proteolytic enzyme (trypsin, chimotrypsin, subtilisin and papain) inhibitors, lectins, and tannins in the resistance mechanisms. Fractionation of the seed meal of a resistant variety suggests that the factor(s) responsible for the effect is (are) concentrate in the globulin fraction.