FEMS Microbiology Letters · 2001 · 24 citations · 23 references
Life SciencesGeneticsMicrobiologyExperimental EvolutionMicrobial EvolutionMicrobial GeneticsBacterial EvolutionBacterial GeneticsGenotypic CharacterizationMicrobial Population GeneticsMicrobial Population HeterogeneityMolecular Population GeneticsBacterial Genetic VariationBacterial MutationBacterial Population GeneticsGel ElectrophoresisGenetic Variability AnalysisPolyacrylamide Gel ElectrophoresisProtein Content DeterminationPulsed-field Gel Electrophoresis
The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) even after decades of undisturbed storage. Considerable genetic variability was observed in these populations. Three genetic variables, chromosome fragment size as determined by pulsed-field gel electrophoresis, extensive mutational reversions from nutritional auxotrophy to prototrophy, and differences in protein content as assayed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, were measured.
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High Mutation Frequencies Among <i>Escherichia coli</i> and <i>Salmonella</i> Pathogens
J. Eugene LeClerc, Baoguang Li, W L Payne et al. · Science · 1996 · 846 citations