Convergent Chemical Synthesis of Proteins by Ligation of Peptide Hydrazides

Abstract

Coming together: A generally applicable strategy for convergent chemical synthesis of proteins from multiple peptide segments is developed on the basis of the ligation of peptide hydrazides. The peptide hydrazide intermediates can be made at low cost and the new strategy is used in the synthesis of the 142 residue model protein RpS25 from six peptide segments. PG=protecting group.