Solid‐Phase Enzyme Activity Assay Utilizing an Entrapped Fluorescence‐Signaling DNA Aptamer

Abstract

Layered enzyme assays: The use of a layered sol-gel based material allows for spatial separation of coimmobilized enzyme and fluorescent aptamer reporter species. This provides a route for facile assaying of enzyme activity and inhibition in cases where the aptamer signals the presence of the enzyme substrate. This then results in accurate IC50 determinations.