Investigation of Selected Immunocytogenetic Effects of Wet Cupping in Healthy Men

Abstract

SUMMARY AIM: This present study was carried out to evaluate effect of cupping therapy using selected immunocytogenetic parameters. METHODS: Forty four males participated as two groups; group I (age 21 ± 1 year) of 30 persons (23 experimental, 7 control) and group II (age over 40 years) of 14 subjects (9 experimental and 5 control). The experimental group received cupping treatment while the control group did not. Basic wet cupping was performed by a practitioner. Peripheral blood samples were collected over a month period; one day before cupping, one week after cupping and a third one month follow up sample was obtained to determine longer term carryover of the possible effects of cupping. White and red blood cells (WBC and RBC) count and hemoglobin (Hb) concentration were measured. 50% Hemolytic Complement (CH50) activity was conducted. Chromosomes were prepared using a colchicines-fixative-air drying-Giemsa schedule. The Cell Replication Index (CRI) and Sister Chromatid Exchanges (SCE) frequencies were recorded. RESULTS: Levels of SCE did not significantly differ between experimental and control groups. The same applied to CRI. Although WBC counts were significantly higher in persons after wet cupping, this was not the case for the number of RBCs and Hb concentration. Complement activity was enhanced by cupping in the older group but not in the younger one. CONCLUSIONS: No correlation between cupping and cytogenetic parameters (CRI and SCE) was observed. However, cupping seems to play a role in activation of complement system as well as modulation of cellular part of immune system.