Haematin-dependent Oxidative Phosphorylation in Streptococcus faecalis

Abstract

SUMMARY Functional cytochrome(s) and an oxidative phosphorylation system were demonstrated in Streptococcus faecalis grown on media supplemented with haematin. The membrane fraction of cell-free extracts coupled the oxidation of reduced NAD to the formation of ATP from ADP and in organic phosphate. This reaction was sensitive to cyanide, azide and Antimycin a. A b 2 type cytochrome was detected in the membrane fraction with absorption maxima at 412, 425, 529 and 559 nm.