Development of a 5′ Fluorogenic Nuclease-Based Real-Time PCR Assay for Quantitative Detection of <i>Actinobacillus actinomycetemcomitans</i> and <i>Porphyromonas gingivalis</i>

Abstract

A 5' nuclease TaqMan PCR was developed for the quantitative detection of the periodontopathic bacteria Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The relative numbers of bacteria were measured by the comparative threshold cycle method. This simplified method is a way of obtaining the relative quantities of these organisms from specimens and of monitoring the effect of therapy.