Orthogonal Alkynyl Amino Acid Reporter for Selective Labeling of Bacterial Proteomes during Infection

Abstract

Bacterial proteins in the haystack: The alkynyl amino acid reporter AOA (see scheme) can be used to visualize and identify newly synthesized bacterial proteins during Salmonella infection of mammalian cells by the copper(I)-catalyzed azide–alkyne cycloaddition. This technique opens new opportunities for the system-wide analysis of bacterial pathogens.