Search for Breast Cancer Biomarkers in Fractionated Serum Samples by Protein Profiling With SELDI‐TOF MS

Abstract

By extensive serum fractionation, we detected many more proteins than in previous studies without fractionation. However, discriminating proteins were still high abundant. Results indicate that either lower abundant proteins are less distinctive, or more rigorous fractionation and selective protein depletion, or a more sensitive assay, are needed to detect lower abundant discriminative proteins.