Nucleic Acids Research · 1981 · 287 citations · 7 references
Natural SciencesChemistryMolecular BiologyNucleic Acid BiochemistryNucleic Acid ChemistrySpectroscopyDna ChemistryDna StructureNucleic Acid StructurePolymer CharacterizationPolymer StructureRaman SpectroscopyAqueous SolventsCrystalline StructureNucleic Acid SpectroscopyRaman SpectraRaman Spectral InterpretationSalt Effects On Base PairingSpectral CharacteristicsZ-dna
The laser-Raman spectra of crystalline d(CpGpCpGpCpG) and of aqueous poly(dG-dC).poly(dG-dC) in high salt (4M NaCl) and low salt (0.1M NaCl) solutions have been measured and compared. The spectra of the crystal and the high-salt solution show a striking congruence, which indicates clearly that the high-salt form of the aqueous polymer has the left-handed Z-DNA structure of the crystalline oligomer. These two spectra differ substantially from that of the low-salt form of the polymer, which has been found previously to have spectral characteristics of the B-form of DNA. The high salt spectrum shows a unique line due to guanine residues at 625 cm-1 which should be useful for qualitative and possibly quantitative assessment of the amount of Z-structure present in a sample of DNA.
7
Molecular structure of a left-handed double helical DNA fragment at atomic resolution
Andrew H.‐J. Wang, Gary J. Quigley, Francis J. Kolpak et al. · Nature · 1979 · 2.1K citations
Left-Handed Double Helical DNA: Variations in the Backbone Conformation
Andrew H.‐J. Wang, Gary J. Quigley, Francis J. Kolpak et al. · Science · 1981 · 575 citations