Microsphere‐Based Real‐Time Calcium Sensing

Abstract

Sensing within the cell: Microspheres (2 μm) covalently loaded with calcium sensors can be delivered efficiently into living cells to analyze the intracellular changes in the concentration of Ca2+ ions in real time. The figure shows the real-time ratiometric fluorescent analysis (400/475 nm ratio) of the release of Ca2+ ions in cells loaded with the microspheres.