Microsphere‐Based Real‐Time Calcium Sensing

Abstract

Sensing within the cell: Microspheres (2 μm) covalently loaded with calcium sensors can be delivered efficiently into living cells to analyze the intracellular changes in the concentration of Ca2+ ions in real time. The figure shows the real-time ratiometric fluorescent analysis (400/475 nm ratio) of the release of Ca2+ ions in cells loaded with the microspheres. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z601242_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.