Protein and Peptide Letters · 2008 · 18 citations · 0 references
Life SciencesBiochemistryMolecular BiologyMolecular NeurosciencePost-translational ModificationProteomicsCerebrospinal Fluid BiomarkersClinical ProteomicsMass Spectrometry-based ProteomicsPost-translational Modification AnalysisCerebrospinal Fluid AnalysisCerebrospinal Fluid MarkersDisease ProteomicsPost-translational Modification SitesTwo-dimensional ElectrophoresisTwo-dimensional Gel Electrophoresis
During analysis of the proteome in the cerebrospinal fluid (CSF) of the Caucasian form of moyamoya disease (MMD), a novel post-translational modification of human transthyretin was observed. Two-dimensional electrophoresis and subsequent peptide sequencing with ESI-MS/MS were performed to discover the γ-carboxylation of the Glu-42 (Gla- 42). Keywords: γ-Carboxy-glutamate, cerebrospinal fluid, moyamoya disease, post-translational modification, human transthyretin, two-dimensional gel electrophoresis