The Infectivity of Polyoma Virus DNA for Mouse Embryo Cells in the Presence of Diethylaminoethyl-dextran

Abstract

SUMMARY Plaques were produced in mouse-embryo monolayers by infection with polyoma virus DNA in the presence of diethylaminoethyl-dextran. Optimum conditions for plaque assay were established and dose-response relationships for component I and component (II+III) polyoma DNA determined. Efficiencies up to 6 × 105 p.f.u./µg. were obtained for component I DNA.