Molecular Microbiology · 2010 · 23 citations · 30 references
Life SciencesMicrobiologyMolecular BiologyCell DivisionCytoskeletonMicrobial GeneticsActin FilamentsActin PolymerizationBacterial Gene RegulationBacterial SporulationMacromolecular AssemblyMicrobial DivisionBacterial Cell DivisionBacterial Sporulation RegulationCell Division ControlChromosome Segregation During SporulationInteraction Partner IdentificationMitotic Chromosome SegregationPar ProteinsPolar Growth
Bacterial chromosome segregation usually involves cytoskeletal ParA proteins, ATPases which can form dynamic filaments. In aerial hyphae of the mycelial bacterium Streptomyces coelicolor, ParA filaments extend over tens of microns and are responsible for segregation of dozens of chromosomes. We have identified a novel interaction partner of S. coelicolor ParA, ParJ. ParJ negatively regulates ParA polymerization in vitro and is important for efficient chromosome segregation in sporulating aerial hyphae. ParJ-EGFP formed foci along aerial hyphae even in the absence of ParA. ParJ, which is encoded by sco1662, turned out to be one of the five actinobacterial signature proteins, and another of the five is a ParJ paralogue. We hypothesize that polar growth, which is characteristic not only of streptomycetes, but even of simple Actinobacteria, may be interlinked with ParA polymer assembly and its specific regulation by ParJ.
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Bertolt Gust, Gregory L. Challis, Kay Fowler et al. · Proceedings of the National Academy of Sciences · 2003 · 1.4K citations