Clinical Chemistry · 1957 · 22 citations · 0 references
Natural SciencesBiochemistryAnalytical ChemistryChromatographic AnalysisElectroanalytical MethodsAnalytical ChromatographyChromatographyProtein ElectrophoresisDenaturationElectrophoresisPaper ElectrophoresisPh Dependence
Abstract Practical physical and chemical procedures available for the identification of hemoglobins have been discussed. These include electrophoresis, chromatography, alkali denaturation, and ferrohemoglobin solubility. The conditions essential for satisfactory electrophoretic separations have been considered and two procedures for paper electrophoresis at pH 6.5, 7.8, and 8.6 using apparatus for compressed paper strips and for freely suspended horizontal strips are presented. A method for the determination of ferrohemoglobin solubility also has been described.