Label‐Free Detection of Protein–Protein Interactions on Biochips

Abstract

Eliminating the middle-man: The combination of MALDI-TOF mass spectrometry and self-assembled monolayers on gold (SAMDI) offers a new detection method for protein–ligand (shown) and protein–protein interactions. Proteins and protein complexes are selectively bound to biochips and then directly analyzed by MALDI-TOF MS without the need for labels. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z501363_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.