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Purification and Partial Characterization of Tryptophan 7-Halogenase (PrnA) fromPseudomonas fluorescens
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2000
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Aldo-keto ReductaseBiochemistryReactive Nitrogen SpecieMedicineNatural SciencesMicrobial ProteomicsEnzyme CatalysisFlavin ReductaseMolecular BiologyTryptophan 7-HalogenaseStructure-function Enzyme KineticsMicrobiologySecond ProteinRedox BiologyOxidative Stress
In an oxygen-dependant reaction, tryptophan 7-halogenase (PrnA) regioselectively chlorinates tryptophan (1) at the 7-position to yield 2. During purification of the halogenase, a flavin reductase, as second protein component, was detected in addition to NADH and FAD; this second protein is also required for the reaction.