Ocular Immunology and Inflammation · 2013 · 53 citations · 40 references
MedicineNeurobiologyNeuroscienceCell TherapyRegenerative MedicineStem Cell BiologyInner Retinal FunctionMesenchymal Stem Cell TherapyMesenchymal Stromal Cell TherapyRetinal DegenerationRetinal Ganglion CellsRetinal NeurobiologyImmunomodulatory EffectsRetinal Disease ModelsRetinal NeuroprotectionRetinal Stem Cells
PURPOSE: To investigate the neuroprotective and immunomodulatory effects of mesenchymal stem cells (MSCs) engineered to secrete CX3CL1 on the light-injured retinal structure and function. METHODS: Normal MSCs and CX3CL1-expressing MSCs (CX3CL1-MSCs) were transplanted into the subretinal space of light-injured rats. By ERG and TUNEL methods, their rescue effect of the host retina was compared with untreated light-injured and vehicle-injected rats. Activated microglia in the retina were stained by ED-1 antibody, and Western blot was performed to quantify cytokines secreted by the retina post-transplantation. RESULTS: ERG analysis showed better function in CX3CL1-MSC-injected group than other groups at 21 days after transplantation (p < 0.05). CX3CL1-MSCs inhibited apoptosis of the retinal cells and microglial activation. Neurotrophic factors expression in host retina that received CX3CL1-MSCs was stronger than in the retina that received normal MSCs. Conversely, the expression of proinflammatory factors was downregulated. CONCLUSIONS: CX3CL1-MSCs subretinal transplantation may enhance protective effect against light-induced retinal degeneration.
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