A Molecular Beacon‐Based Signal‐Off Surface‐Enhanced Raman Scattering Strategy for Highly Sensitive, Reproducible, and Multiplexed DNA Detection

Abstract

Stem-loop oligonucleotides tagged with organic dyes are employed as capture and reporter probes in a molecular beacon-based signal-off surface- enhanced Raman scattering (SERS) strategy. DNA strands are immobilized on a gold nanoparticle-decorated silicon nanowire array ([email protected]). DNA with a concentration down to ≈10 fM is detected under irradiation from a low-power laser, which is better than or comparable to that detected by signal-on SERS methods. The signal-off strategy allows identification of single-base mismatches and detection of multiplexed DNA. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.