Rapid Detection of Enterohemorrhagic <i>Escherichia coli</i> by Real-Time PCR with Fluorescent Hybridization Probes

Abstract

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.