Analysis of Solid-Phase Reactions: Product Identification and Quantification by Use of UV-Chromophore-Containing Dual-Linker Analytical Constructs

Abstract

Detection and quantification of material synthesized on a single resin bead is now possible using standard UV spectroscopy and mass spectrometry by the introduction of an analytical construct (A) before the linker (L2; see scheme). Cleavage of the analytical fragment is achieved by the introduction of another, orthogonal, linker (L1) that releases the labeled substrate into solution.